abstract：:Protein expression of Spodoptera frugiperda (Sf9) insect cells was characterized upon exposure to environmental stresses typically present in bioreactors including heat shock, oxygen deprivation, shear stress, change of pH, and salinity or ethanol shock. This study fills the void in knowledge as to how bioreactor hydr...

journal_title：Journal of biotechnology

authors： Huhtala A,Linko P,Mutharasan R

更新日期：2005-08-22 00:00:00

abstract：:Owing to its dual impact on tissue engineering (neovascularization of tissue implants) and cancer treatment (prevention of tumor-induced vascularization), management and elucidation of vascularization phenomena remain clinical priorities. Using a variety of primary human cells and (neoplastic) cell lines assembled in ...

journal_title：Journal of biotechnology

authors： Kelm JM,Diaz Sanchez-Bustamante C,Ehler E,Hoerstrup SP,Djonov V,Ittner L,Fussenegger M

更新日期：2005-08-04 00:00:00

abstract：:A novel self-regulating bioreactor concept for sulfate reduction is proposed aiming for high biomass concentrations and treatment capacities. The system consists of a cell suspension of sulfate reducing bacteria in a continuous stirred tank reactor (30 degrees C) fed with a mixture of both electron donor and electron ...

journal_title：Journal of biotechnology

authors： Paulo PL,Kleerebezem R,Lettinga G,Lens PN

更新日期：2005-07-21 00:00:00

abstract：:Escherichia coli overexpressing a NAD(+)-dependent formate dehydrogenase (FDH) from Candida boidinii was grown in chemostat culture on various carbon sources at 0.05 h(-1) dilution rate, under anaerobic conditions using defined medium and compared to a control without the heterologous FDH pathway. Metabolic fluxes, NA...

journal_title：Journal of biotechnology

authors： Sánchez AM,Bennett GN,San KY

更新日期：2005-06-29 00:00:00

abstract：:Three matrices were used for immobilizing the cytochrome c: Sepharose CL-4B, Silasorb SPH amine and a laboratory-prepared new matrix based on crosslinked triazine (2,4,6-tris(aminoethylamine)-1,3,5-triazine) (TAT). Cytochrome c was immobilized on the matrices by several procedures and the amount of incorporated cytoch...

journal_title：Journal of biotechnology

authors： Janiczek O,Pokorna B,Zemanova J,Mandl M

更新日期：2005-05-25 00:00:00

abstract：:Two different approaches of matrix assisted refolding have been evaluated and compared to conventional refolding by dilution. Bovine alpha-lactalbumin was used for the studies as model protein. It was adsorbed under denaturing conditions on an ion exchange matrix and refolding was completed on the column prior to elut...

journal_title：Journal of biotechnology

authors： Machold C,Schlegl R,Buchinger W,Jungbauer A

更新日期：2005-04-20 00:00:00

abstract：:Three thermostable lactose-hydrolases, namely, two beta-glycosidases (bglA and bglB) and one beta-galactosidase (bgaA) genes were cloned from the genomic library of Thermus sp. IB-21. The bglA, bglB, and bgaA consisted of 1311 bp (436 amino acid residues), 1296 bp (431 aa), and 1938 bp (645 aa) of nucleotides with pre...

journal_title：Journal of biotechnology

authors： Kang SK,Cho KK,Ahn JK,Bok JD,Kang SH,Woo JH,Lee HG,You SK,Choi YJ

更新日期：2005-04-06 00:00:00

abstract：:Enzymatic synthesis of acylated derivatives of a monosaccharidic flavonoid chrysoeriol-7-O-beta-D-(3''-E-p-coumaroyl)-glucopyranoside as well as of a disaccharidic flavonoid chrysoeriol-7-[6'''-O-acetyl-beta-D-allosyl-(1-->2)-beta-D-glucopyranoside], isolated from Greek endemic plants, was performed using an immobiliz...

journal_title：Journal of biotechnology

authors： Mellou F,Lazari D,Skaltsa H,Tselepis AD,Kolisis FN,Stamatis H

更新日期：2005-03-30 00:00:00

abstract：:The use of the fungus Aspergillus niger for the bioleaching of heavy metals from spent catalyst was investigated, with fluid catalytic cracking (FCC) catalyst as a model. Bioleaching was examined in batch cultures with the spent catalysts at various pulp densities (1-12%). Chemical leaching was also performed using mi...

journal_title：Journal of biotechnology

authors： Aung KM,Ting YP

更新日期：2005-03-16 00:00:00

abstract：:Pellet growth of Aspergillus terreus ATCC 20542 in submerged batch fermentations in stirred bioreactors was used to examine the effects of agitation (impeller tip speed u(t) of 1.01-2.71 ms(-1)) and aeration regimens (air or an oxygen-enriched mixture containing 80% oxygen and 20% nitrogen by volume) on the fungal pel...

journal_title：Journal of biotechnology

authors： Casas López JL,Sánchez Pérez JA,Fernández Sevilla JM,Rodríguez Porcel EM,Chisti Y

更新日期：2005-03-02 00:00:00

abstract：:The aim of this study is to evaluate the utility of using aerobic acetate-fed microbial granules as a starting seed to rapidly develop stable aerobic phenol-degrading granules. Aerobic granules were first cultivated in four sequencing batch reactors with acetate as sole carbon source at a loading rate of 3.8 kg m(-3) ...

journal_title：Journal of biotechnology

authors： Tay ST,Moy BY,Jiang HL,Tay JH

更新日期：2005-02-23 00:00:00

abstract：:Aliphatic thiols are effective as redox buffers for folding non-native disulfide-containing proteins into their native state at high pH values (8.0-8.5) but not at neutral pH values (6-7.5). In developing more efficient and flexible redox buffers, a series of aromatic thiols was analyzed for its ability to fold scramb...

journal_title：Journal of biotechnology

authors： Gough JD,Lees WJ

更新日期：2005-02-09 00:00:00

abstract：:Preparations enriched by a specific protein are rarely easily obtained from natural host cells. Hence, recombinant protein production is frequently the sole applicable procedure. The ribosomal machinery, located in the cytoplasm is an outstanding catalyst of recombinant protein biosynthesis. Escherichia coli facilitat...

journal_title：Journal of biotechnology

authors： Sørensen HP,Mortensen KK

更新日期：2005-01-26 00:00:00

abstract：:A simple and an efficient oligonucleotide array was developed to identify common severe determinants of alpha (alpha) thalassemia. A total of 14 probes were designed to detect the most frequently three deletions (-alpha(3.7), -alpha(4.2), -(SEA)) and two non-deletions (alpha(Quong Sze), alpha(Constant Spring)). PCR pr...

journal_title：Journal of biotechnology

authors： Ye BC,Zhang Z,Lei Z

更新日期：2005-01-12 00:00:00

abstract：:The effect of an additional carbon source, lactate, on Penicillium camembertii growth on glutamate as both carbon and nitrogen sources was examined. Glutamate (and lactate) was present in excess in both media. Throughout the whole culture, similar growth time-courses were recorded on both media, indicating the absence...

journal_title：Journal of biotechnology

authors： Adour L,Couriol C,Amrane A

更新日期：2004-11-09 00:00:00

abstract：:We cloned and characterized a preprochymosin cDNA from the abomasum of milk-fed kid goats. This cDNA contained an open reading frame that predicts a polypeptide of 381 amino acid residues, with a signal peptide and a proenzyme region of 16 and 42 amino acids, respectively. Comparison of the caprine preprochymosin sequ...

journal_title：Journal of biotechnology

authors： Vega-Hernández MC,Gómez-Coello A,Villar J,Claverie-Martín F

更新日期：2004-10-19 00:00:00

abstract：:Baculovirus vectors show promise as a novel tool for gene delivery into mammalian cells and gene transfer with wild-type baculovirus has been demonstrated both in vitro and in vivo. To study expression and intracellular trafficking of foreign viral membrane proteins in baculovirus-transduced mammalian cells, the envel...

journal_title：Journal of biotechnology

authors： Ojala K,Tikka PJ,Kautto L,Käpylä P,Marjomäki V,Oker-Blom C

更新日期：2004-10-19 00:00:00

abstract：:Enzyme engineering by molecular modelling and site-directed mutagenesis can be remarkably efficient. Directed enzyme evolution appears as a more general strategy for the isolation of catalysts as it can be applied to most chemical reactions in aqueous solutions. Selections, as opposed to screening, allow the simultane...

journal_title：Journal of biotechnology

authors： Jestin JL,Kaminski PA

更新日期：2004-09-30 00:00:00

abstract：:Dual nutrient limited growth, the control of the cell growth rate (kinetic aspect) or the restriction of the amount of biomass (stoichiometric aspect) by two nutrients at the same time, is a relatively unknown ability of the microorganisms and consequently, still not mentioned in textbooks to date. Nevertheless, multi...

journal_title：Journal of biotechnology

authors： Zinn M,Witholt B,Egli T

更新日期：2004-09-30 00:00:00

abstract：:Paramagnetic carriers, which are linked to antibodies enable highly specific biological cell separations. With the colloidal synthesis of superparamagnetic Co and FeCo nanocrystals with superior magnetic moments the question about their potential to replace magnetite as the magnetically responsive component of magneti...

journal_title：Journal of biotechnology

authors： Hütten A,Sudfeld D,Ennen I,Reiss G,Hachmann W,Heinzmann U,Wojczykowski K,Jutzi P,Saikaly W,Thomas G

更新日期：2004-08-26 00:00:00

abstract：:Using an RT-PCR approach a cDNA clone, designated Ms-Rac4 and putatively coding for a small GTPase was isolated from Medicago sativa. Ms-Rac4 and the earlier described Ms-Rac1 [Mol. Gen. Genet. 263 (2000) 761] belong to the class of GTP-binding Rho of plants (Rop) proteins. At the amino acid level they display all con...

journal_title：Journal of biotechnology

authors： Brecht M,Sewald K,Schiene K,Keen G,Fricke M,Sauer M,Niehaus K

更新日期：2004-08-26 00:00:00

abstract：:A transgenic tobacco plant (Nicotiana tabacum L.) expressing a fungal pectin methylesterase (PME; EC 3.1.1.11) gene derived from a black filamentous fungus, Aspergillus niger was created. Fungal PME should have a wider range of adaptability to substrate pectin compared with plant PME. As expected, the proportion of me...

journal_title：Journal of biotechnology

authors： Hasunuma T,Fukusaki E,Kobayashi A

更新日期：2004-08-05 00:00:00

abstract：:Stem borer resistant transgenic parental lines, involved in hybrid rice, were produced by Agrobacterium-mediated gene transfer method. Two pSB111 super-binary vectors containing modified cry1Ab/cry1Ac genes driven by maize ubiquitin promoter, and herbicide resistance gene bar driven by cauliflower mosaic virus 35S pro...

journal_title：Journal of biotechnology

authors： Ramesh S,Nagadhara D,Pasalu IC,Kumari AP,Sarma NP,Reddy VD,Rao KV

更新日期：2004-07-15 00:00:00

abstract：:The extraction of 13-hydroperoxide-lyase activity from mint leaves as well as its use for C6-aldehyde production was studied in this work. The enzyme cleaves 13(S)-hydroperoxy-C18 fatty acids into C6-aldehyde and C12-oxo-acid. Two mint species were tested: Mentha veridis and Mentha pulegium. The headspace injection me...

journal_title：Journal of biotechnology

authors： Gargouri M,Drouet P,Legoy MD

更新日期：2004-07-01 00:00:00

abstract：:The use of recombinant proteins has increased greatly in recent years, as well as the techniques used for their purification. The selection of an efficient process to purify proteins is a major bottleneck found when trying to scale up results obtained in the laboratory to a large-scale industrial process. One of the m...

journal_title：Journal of biotechnology

authors： Vásquez-Alvarez E,Pinto JM

更新日期：2004-06-10 00:00:00

abstract：:One potential reason for the enhancement of immune responses to DNA vaccines following electroporation is increased gene expression. However, the inflammatory response and accompanying cellular infiltration stimulated by electroporation may also be essential for enhancing immune responses to DNA vaccines. These parame...

journal_title：Journal of biotechnology

authors： Babiuk S,Baca-Estrada ME,Foldvari M,Middleton DM,Rabussay D,Widera G,Babiuk LA

更新日期：2004-05-13 00:00:00

abstract：:Two extra-cellular endoxylanases (Xyl Ia and Ib) were purified to homogeneity from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099. Xyl Ia and Ib, having a molecular mass of approximately 53 kDa and pI of 5.2 and 4.8, respectively, were optimally active at 75 degrees C and at pH 6.0. They were st...

journal_title：Journal of biotechnology

authors： Chadha BS,Ajay BK,Mellon F,Bhat MK

更新日期：2004-04-29 00:00:00

abstract：:The secreted pore-forming toxin listeriolysin O (LLO) is an essential virulence factor that allows the food-borne bacterial pathogen Listeria monocytogenes to escape from the phagocytic vacuole and reach the host cytosol. This protein belongs to the group of cholesterol-binding sulfhydryl-activated toxins, expressed b...

journal_title：Journal of biotechnology

authors： Giammarini C,Andreoni F,Amagliani G,Casiere A,Barocci S,Magnani M

更新日期：2004-04-08 00:00:00

abstract：:Superoxide dismutases are ubiquitous enzymes which play an important role in protecting cells against oxidative damage and which have also been shown to contribute to the pathogenicity of many bacterial species. Here we demonstrate that Vibrio cholerae, the causative agent of cholerae, expresses an active periplasmic ...

journal_title：Journal of biotechnology

authors： Gabbianelli R,Signoretti C,Marta I,Battistoni A,Nicolini L

更新日期：2004-04-08 00:00:00

abstract：:A high number of economically important recombinant proteins are produced in Escherichia coli based host/vector systems. The major obstacle for improving current processes is a lack of appropriate on-line in situ methods for the monitoring of metabolic burden and critical state variables. Here, a pre-evaluation of the...

journal_title：Journal of biotechnology

authors： Reischer H,Schotola I,Striedner G,Pötschacher F,Bayer K

更新日期：2004-03-04 00:00:00

abstract：:Supervision of batch bioprocess operations in real-time during the progress of a batch run offers many advantages over end-of-batch quality control. Multivariate statistical techniques such as multiway partial least squares (MPLS) provide an efficient modeling and supervision framework. A new type of MPLS modeling tec...

journal_title：Journal of biotechnology

authors： Undey C,Tatara E,Cinar A

更新日期：2004-02-19 00:00:00

abstract：:Aspergillus sojae, which is believed to be a domesticated strain of Aspergillus parasiticus, contains all of the aflatoxin biosynthetic genes but is unable to produce aflatoxins and is generally recognized as safe (GRAS) for producing fermented foods. In A. parasiticus both aflR, the aflatoxin pathway-specific regulat...

journal_title：Journal of biotechnology

authors： Chang PK

更新日期：2004-02-05 00:00:00

abstract：:When subjected to a Picea abies suspension cell culture, beta-pinene, either one of the pure enantiomers or the racemate, was transformed mainly to trans-pinocarveol along with the minor products myrtenol, alpha-terpineol, pinocarvone, myrtenal and cis-pinocarveol. The absolute configuration of the major products corr...

journal_title：Journal of biotechnology

authors： Lindmark-Henriksson M,Isaksson D,Vanek T,Valterová I,Högberg HE,Sjödin K

更新日期：2004-01-22 00:00:00

abstract：:Flax (Linum usitatissimum L.) is an annual plant species widely cultivated in temperate climates for bast fibres and linseed oil. Apart from traditional textile use, the fibres are fast becoming an integral part of new composite materials utilized in automobile and constructive industry. Especially attractive for envi...

journal_title：Journal of biotechnology

authors： Wróbel M,Zebrowski J,Szopa J

更新日期：2004-01-08 00:00:00

abstract：:Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete. It infects tomato, spreads through the xylem and causes bacterial wilt and canker. The wild-type strain NCPPB382 carries two plasmids, pCM1 and pCM2. The cured plasmid-free derivative CMM100 is still able to colonize tomato, but no dise...

journal_title：Journal of biotechnology

authors： Gartemann KH,Kirchner O,Engemann J,Gräfen I,Eichenlaub R,Burger A

更新日期：2003-12-19 00:00:00

abstract：:A macrokinetic model for Pichia pastoris expressing recombinant human serum albumin is proposed. The model describes the balances of some key metabolites, ATP and NADH, during glycerol and methanol metabolism. In the glycerol growth phase, the metabolic pathways mainly include phosphorylation, glycolysis, tricarboxyli...

journal_title：Journal of biotechnology

authors： Ren HT,Yuan JQ,Bellgardt KH

更新日期：2003-12-05 00:00:00

abstract：:Propenylbenzenes are often used as starting materials in the chemical synthesis of aroma compounds and fine chemicals. In the present study, we demonstrate the ability of an Arthrobacter sp. to transform various structures of propenylbenzenes derived from essential oils to flavor, fragrance, and fine chemicals. Arthro...

journal_title：Journal of biotechnology

authors： Shimoni E,Baasov T,Ravid U,Shoham Y

更新日期：2003-10-09 00:00:00

abstract：:Osmoregulation, the adaptation of cells to changes in the external osmolarity, is an important aspect of the bacterial stress response, in particular for a soil bacterium like Corynebacterium glutamicum. Consequently, this organism is equipped with several redundant systems for coping with both hyper- and hypoosmotic ...

journal_title：Journal of biotechnology

authors： Morbach S,Krämer R

更新日期：2003-09-04 00:00:00

abstract：:The genome sequence of Corynebacterium glutamicum, a gram-positive soil bacterium widely used as an amino acid producer, was analyzed by a similarity-based approach to elucidate the pathway for the biosynthesis of L-methionine. The functions of candidate ORFs were derived by gene deletion and, if necessary, by homolog...

journal_title：Journal of biotechnology

authors： Rückert C,Pühler A,Kalinowski J

更新日期：2003-09-04 00:00:00

abstract：:A computer system solution for integration of a distributed bioreactor monitoring and control instrumentation on the laboratory scale is described. Bioreactors equipped with on-line analyzers for mass spectrometry, near-infrared spectroscopy, electrochemical probes and multi-array gas sensors and their respective soft...

journal_title：Journal of biotechnology

authors： Cimander C,Bachinger T,Mandenius CF

更新日期：2003-08-15 00:00:00